Check out using a credit card or bank account with. Some 3,617 A. dorsata genes did not have orthologs in any of the included Apis species. In some countries, there are strict quarantine requirements for giant honey bees as they may carry parasitic mites which will affect badly for the local honey bee industry. This item is part of JSTOR collection

Uncovering the novel characteristics of Asian honey bee, Biomedical activity and related volatile compounds of Thai honeys from 3 different honeybee species, Midgut bacterial communities in the giant Asian honeybee (, Allozyme variation and differentiation among four Apis species, BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs, Automated generation of heuristics for biological sequence comparison, Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee, Honeybees and other insect pollinators of cultivated plants: a review, Evolutionary ancient roles of serotonin: long-lasting regulation of activity and development. pollination purposes. Access supplemental materials and multimedia. Variation in the activity of Lactobacillus strains in honeybee microbiomes can affect the flavor of honey since flavor is partially dependent on lactic acid bacterial metabolites (Olofsson et al. The enriched InterPro signature IPR001127, a domain that is associated with the carbohydrate transport system in Lactobacillus bacteria, was found in six of the species-specific A. dorsata genes. In addition, A. dorsata is distinguished by a lack of body size variation between castes as in other honey bees. honey bee species, Apis dorsata honey contains high moisture and low sugar Supplementary data are available at Genome Biology and Evolution online. The average effective number of matings per queen was 25.56 ± 11.63. Although three of these had orthologs in other Apis species, one was sufficiently divergent that it did not cluster with any other Apis genes. Extracted DNA was sheared to 20 kb using a Covaris G-tube following manufacturer’s recommendations. The sheared DNA was prepared for Nanopore sequencing using the Ligation Sequencing 1D kit (SQK-LSK108). Three of these were potentially drifted offspring workers from genotyped queens of colonies nesting on the same tree. Origin of primate orphan genes: a comparative genomics approach.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, Large-scale phylogenetic analysis of trypanosomatid adenylate cyclases reveals associations with extracellular lifestyle and host-pathogen interplay, The influence of chromosomal environment on X-linked gene expression in, Mitochondrial DNA Sequence Diversity in Mammals: A Correlation between the Effective and Census Population Sizes, Dorsal Pigmentation and Its Association with Functional Variation in, Evolutionary and Comparative analysis of bacterial Non-Homologous End Joining Repair, Volume 12, Issue 10, October 2020 (In Progress), About the Society for Molecular Biology and Evolution, https://github.com/sanger-pathogens/assembly-stats, http://creativecommons.org/licenses/by-nc/4.0/, Receive exclusive offers and updates from Oxford Academic, Copyright © 2020 Society for Molecular Biology and Evolution. For terms and use, please refer to our Terms and Conditions © 1995 Springer While much of this variation can be explained by the nectar source that bees use to produce the honey, honey characteristics also differ based on honeybee species. 2004), has limited the ability of humans to domesticate the species and to commercially produce its honey (Koeniger et al. We identified four 5-HT2A receptors in A. dorsata (fig. Giant honey bees are eusocial insects with the characteristics like cooperative brood care, overlapping generations within a colony of adults and reproductive caste …

The time taken for an egg to develop into an adult differs between different castes (table 1) 14. The flowcell was sequenced with standard parameters for 2 days. Our assembly results were consistent with previous Apis genome sequencing projects (fig. Species-specific genes were narrowly defined as sequences that lacked orthologs in any other Apis species. Mol Biol Evol 26(7):1641–1650. After a final Ampure clean-up, the library was loaded on to a MinION R9.0 SpotON flowcell as per manufacturer’s instructions. Zdobnov EM. 2A and B). (2014). 2011). Hachette UK: Black Dog & Leventhal Publishers. 2004). High degree of polyandry in Apis dorsata queens detected by DNA microsatellite variability Received:21 January 1995/Accepted after revision: 16 July 1995 Abstract Workers of six colonies of the giant honey- bee Apis dorsata from Sabah, Malaysia (five colonies) and Java (one colony) were genotyped using single locus DNA fingerprinting. other true honey bee species, giant honey bee colonies can’t be transported for

Using MAKEr, we identified 13,517 protein coding genes (vs 12,145–12,940 genes in other apids, Consortium 2006; Wang et al. The Asian honey bee (Apis dorsata) is distinct from its more widely distributed cousin Apis mellifera by a few key characteristics. bee colonies are typically large and they are efficient honey producers. In most honey bee species including Apis mellifera, queens and drones are larger than the female worker bees, but there’s only a slight difference in body size between the worker bees and reproductive bees in giant honey bees. Rusch DB Behavioral Ecology and Sociobiology Price MN, Dehal PS, Arkin AP. Lactobacillus species are thought to prevent the fermentation of stored honey by retarding the growth of fermenting yeasts (Olofsson and Vásquez 2008). The flavor of A. dorsata honey differs from that of A. mellifera. Apidologie, Springer Verlag, 2007, 38 (3), pp.221-229. Yon Rhee S Unlike species that nest in enclosed spaces like tree trunks and rock crevices, A. dorsata colonies are unwilling to live in the traditional Langstroth hives used by commercial beekeepers (Koeniger et al. The overlap between these annotations left us with 1,251 genes that lacked functional annotation. Another sets of SNAP and AUGUSTUS models were trained with the new outputs. 2010).

harvests from a single colony for months. 2017). across Asia. The microbiome of A. dorsata may also be involved in the distinctive flavor of the honey they produce. Majority of the time, they leave their own colony to mate with queens from other nearby colonies. Giant honey bees are eusocial insects with the characteristics like cooperative brood care, overlapping generations within a colony of adults and reproductive caste system.

Multiple A. dorsata drones were collected by on the campus of Chiang Mai University (Chiang Mai, Thailand). The remaining 2,253 genes had no hits to other hymenoptera (or only had hits to A. dorsata sequences). 2016. 30(4):772–780. Specimens were sacrificed immediately. Both cladograms (A and C) are based on full-length gene alignments; in the interests of space, only a representative section of the full alignment is shown (B and D). 2016). To examine how genes from A. dorsata have diverged from those of the other examined Apis species, we generated multiple sequence alignments with MAFFT (v7.312) (Katoh and Standley 2013). It also allows beekeepers to remove only the honey head to get multiple 2018). Giant honey bees are considered as pests in Australia. We used the assembly-stats tool (https://github.com/sanger-pathogens/assembly-stats) to determine assembly statistics for the dorsata and other Apis genomes. Simão et al. Giant honey bee colonies are capable of migrating great distances, sometimes up to 200 km. Unlike A. mellifera, which is now found throughout the world, A. dorsata is found only in Asia (Chantawannakul et al. In a giant honey bee colony, drones are produced only during the reproductive season to mate with queens. Singapore: Springer. The Asian honey bee (Apis dorsata) is distinct from its more widely distributed cousin Apis mellifera by a few key characteristics.Most prominently, A. dorsata, nest in the open by forming a colony clustered around the honeycomb, whereas A. mellifera nest in concealed cavities. 2015), to classify the protein-coding genes from A. dorsata, A. mellifera, A. cerana, and A. florea into orthologous clusters. 2008; Kaessmann 2010; Lisch 2013; Göke and Ng 2016), and the overrepresentation of transposable element-related signatures in the genome of A. dorsata, suggests that the evolution of “new” genes may have played a role in the behavioral diversification of A. dorsata from other honeybees. It’s a dry season activity works particularly well in —(A) Cladogram with bootstrap support values and (B) sequence alignment of putative 5-hydroxytryptamine (serotonin) receptor 2A (5-HT2A) genes from Apis dorsata and other Apis species. We found that 1,424 of these genes had a BLAST hit to another hymenopteran species, and an additional 75 had a hit to a nonhymenopteran species. Tropilaelaps mites are originally parasites of the all giant honey bee species including Apis dorsata, Apis breviligula and Apis laboriosa. Apis dorsata is much larger than A. mellifera, and A. dorsata workers are almost twice as long as A. mellifera workers. 2009). More than 80% (11,264) of the predicted A. dorsata genes had matches to other Hymenopteran sequences.

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Following an Ampure clean-up step, prepared fragments were ligated to ONT-specific adapters with the NEB blunt/TA master mix kit. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. Draghici S. Oxford University Press is a department of the University of Oxford. Data deposition: This project has been deposited at NCBI BioProject under the accession PRJNA577936. The trained models were then provided to MAKER and a new set of training models generated from the result used for another round of running MAKER.

A more stringent definition requires a lack of orthologs in any published data set (Tautz and Domazet-Lošo 2011), and we used BLASTp searches against NCBI’s nr database to test whether our narrowly defined species-specific genes were legitimate orphans. Consistent with the idea that single copy universal orthologs represent highly conserved genes, every A. dorsata single copy universal ortholog had a hit to other Hymenoptera in the nr database. We identified a wide variety of repetitive elements (supplementary table 1, Supplementary Material online), and these comprised ∼7% of the genome.

A queen undertook two to four mating flights and after fully mating, she had 5.5 +/- 0.9 (n = 5) million sperm in her spermatheca. These highly conserved genes were enriched for functions related to development, including a growth factor responsible for cell proliferation and differentiation (IPR001111), and juvenile hormone binding proteins (JHBP; IPR010562), which regulates embryogenesis, larval development, and reproductive maturation in the adult forms (supplementary table 2, Supplementary Material online). 2013; Park et al.


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